Image->Type->8-bit,灰度變換
Edit->Invert
Image->Adjust->Threshold,灰度閾值設定,拖動完成後點擊Apply
Process->Binary-Fill Holes填補細胞核的空隙
Process->Binary-Watershed打斷細胞核的重疊部分
Process->Noise->Despekle,去除離群點
Process->Smooth,圖像平滑
Analyze->Analyze Particles,計數