of more than 30 oligosaccharide components was obtained, under optimized conditions, in an analysis time of less than 30 min.超過30寡糖成分，得到優化的下不到30分鐘的分析時間條件。 This represents the first RP-HPLC method that can separate a complex mixture of both small and large sulfated oligosaccharides in a single chromatographic step.這是首次反相高效液相色譜法，可以在壹個單壹的色譜分離步驟的小型和大型硫酸寡糖的復雜混合物。 The heparin-derived oligosaccharides, in this mixture, can also be肝素寡糖，在這種混合物，也可以
separated under a second set of RP-HPIPC conditions using a volatile ion-pairing reagent, tributylammonium acetate, to aid in the recovery of individual sulfated oligosaccharides.下壹個反相HPIPC條件的第二套分開使用揮發性離子對試劑，tributylammonium醋酸，以協助個別硫酸寡糖復蘇。 Moreover, it was possible to replace sodium chloride gradient,此外，有可能取代氯化鈉梯度，
required for eluting highly sulfated oligosaccharides, with a fixed, low concentration of a volatile salt, ammonium acetate, by utilizing an acetonitrile gradient.需要洗脫高度硫酸寡糖，與壹個固定的動蕩鹽，低濃度醋酸銨，利用壹乙腈梯度。 This solvent system might make it possible to directly interface this RP-HPIPC separation with mass spectral analysis.這種溶劑系統，因此可能直接接口這個質譜分析反相HPIPC分離。
2003 Elsevier BV All rights reserved. ?2003埃爾塞維爾BV公司版權所有。Keywords: Ion-pairing reagents; Gradient elution; Oligosaccharides關鍵詞：離子配對試劑;梯度洗脫;寡糖